Publications

bioRxiv

Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo

Dawid S. Zyla, Gillian Zipursky, Roberta Della Marca, Davide Lacarbonara, Gele Niemeyer, Weiwei Peng, Camilla Predella, Kyle Stearns, Cameron Leedale, Jonathan Miller, Daniel Tay, Jonathan Kao, Marissa Acciani, Laura Di Clemente, Anirban Das, Diptiben Parekh, Ruben Diaz Avalos, Gael McGill, N Valerio Dorrello, Kathryn M Hastie, Christopher A Alabi, Joost Snijder, Anne Moscona, Alexander L. Greninger, Stefan Niewiesk, Erica Ollmann Saphire, Matteo Porotto

bioRxiv

Structural and Mechanistic Basis for Antibody Neutralization of the Measles Fusion Protein

Dawid S. Zyla, Roberta Della Marca, Davide Lacarbonara, Gele Niemeyer, Gillian Zipursky, Laura Di Clemente, Gabriella Jonathan-Trakht, Gavreel Kalantarov, Marissa Acciani, Giulia Laterza, Dariia Vyshenska, Kathryn M Hastie, Branka Horvat, Alexander L. Greninger, Stefan Niewiesk, Erica Ollmann Saphire, Matteo Porotto

bioRxiv

Uncovering the features of Measles-targeting human antibodies elicited by the MMR vaccine

Marissa Acciani, Dawid Zyla, Gele Niemeyer, Stephanie Harkins, Diptiben Parekh, Emily Pawlack, Davide LaCarbonara, Stefan Niewiesk, Matteo Porotto, Kathryn M. Hastie, Erica Ollmann Saphire

Science Advances

Human endogenous retrovirus K (HERV-K) envelope structures in pre- and post-fusion by cryo-EM

Tomas Mustelin, Chitra Hariharan, Stephanie Harkins, Roshan Raj Rajamanickam, Kathryn Hastie, Diptiben Parekh, Chen Sun, Patrick Penalosa, Cassandra Yu, Elise Wilson, Ruben Diaz Avalos, Victoria Irene Lewis, Fatemeh Moadab, Jeremy Shek, Erica Ollmann Saphire, Dawid S Zyla, Kelly Christine Lee Shaffer

Cell Reports

Structure of a SARS-CoV-2 spike S2 subunit in a pre-fusion, open conformation

Eduardo Olmedillas, Roshan R. Rajamanickam, Ruben Diaz Avalos, Fernanda Ana-Sosa-Batiz, Dawid Zyla, Michelle A. Zandonatti, Stephanie S. Harkins, Sujan Shresta, Kathryn M. Hastie, Erica Ollmann Saphire

bioRxiv

Human endogenous retrovirus K (HERV-K) envelope structures in pre- and post-fusion by Cryo-EM

Jeremy Shek, Chen Sun, Elise M. Wilson, Fatemeh Moadab, Kathryn M. Hastie, Roshan R. Rajamanickam, Patrick J. Penalosa, Stephanie S. Harkins, Diptiben Parekh, Chitra Hariharan, Dawid S. Zyla, Cassandra Yu, Kelly C.L. Shaffer, Victoria I. Lewis, Ruben Diaz Avalos, Tomas Mustelin, Erica Ollmann Saphire

eLife

How herpes is assembled

Dawid S Zyla

Cell

Intracellular Ebola virus nucleocapsid assembly revealed by in situ cryo-electron tomography

Reika Watanabe, Dawid Zyla, Diptiben Parekh, Connor Hong, Ying Jones, Sharon L. Schendel, William Wan, Guillaume Castillon, Erica Ollmann Saphire

Science

A neutralizing antibody prevents postfusion transition of measles virus fusion protein

Dawid S. Zyla, Roberta Della Marca, Gele Niemeyer, Gillian Zipursky, Kyle Stearns, Cameron Leedale, Elizabeth B. Sobolik, Heather M. Callaway, Chitra Hariharan, Weiwei Peng, Diptiben Parekh, Tara C. Marcink, Ruben Diaz Avalos, Branka Horvat, Cyrille Mathieu, Joost Snijder, Alexander L. Greninger, Kathryn M. Hastie, Stefan Niewiesk, Anne Moscona, Matteo Porotto, Erica Ollmann Saphire

Nature Communications

The assembly platform FimD is required to obtain the most stable quaternary structure of type 1 pili

Dawid S. Zyla, Thomas Wiegand, Paul Bachmann, Rafal Zdanowicz, Christoph Giese, Beat H. Meier, Gabriel Waksman, Manuela K. Hospenthal, Rudi Glockshuber

bioRxiv

Intracellular Ebola Virus nucleocapsid assembly revealed by in situ cryo-electron tomography.

Reika Watanabe, Dawid Zyla, Diptiben Parekh, Connor Hong, Ying Jones, Sharon L. Schendel, Willian Wan, Guillaume Castillon, Erica Ollmann Saphire

Cell Reports

Potent Omicron-neutralizing antibodies isolated from a patient vaccinated 6 months before Omicron emergence

Hastie, K.M., Yu, X., Ana-Sosa-Batiz, F., Zyla, D.S., Harkins, S.S., Hariharan, C., Wasserman, H., Zandonatti, M.A., Miller, R., Maule, E., Kim, K., Valentine, K.M., Shresta, S., Saphire, E.O.

SSRN

Potent, Omicron-Neutralizing Antibodies Isolated From a Patient Vaccinated 6 Months Before Omicron Emergence

Hastie, K.M., Yu, X., Batiz, F.A.S., Zyla, D., Harkins, S.S., Hariharan, C., Wasserman, H., Zandonatti, M.A., Miller, R., Maule, E., Kim, K., Valentine, K., Shresta, S., Saphire, E.O.

Cell Chemical Biology

Structural basis for antibody-mediated neutralization of lymphocytic choriomeningitis virus

Moon-Walker, A., Zhang, Z., Zyla, D.S., Buck, T.K., Li, H., Diaz Avalos, R., Schendel, S.L., Hastie, K.M., Crotty, S., Saphire, E.O.

Science Translational Medicine

A cocktail of protective antibodies subverts the dense glycan shield of Lassa virus

Haoyang Li, Tierra Buck, Michelle Zandonatti, Jieyun Yin, Alex Moon-Walker, Jingru Fang, Anatoliy Koval, Megan L. Heinrich, Megan M. Rowland, Ruben Diaz Avalos, Sharon L. Schendel, Diptiben Parekh, Dawid Zyla, Adrian Enriquez, Stephanie Harkins, Brian Sullivan, Victoria Smith, Onyeka Chukwudozie, Reika Watanabe, James E. Robinson, Robert F. Garry, Luis M. Branco, Kathryn M. Hastie, Erica Ollmann Saphire

bioRxiv

Structural basis for antibody-mediated neutralization of Lymphocytic choriomeningitis virus

Alex Moon-Walker, Zeli Zhang, Dawid S. Zyla, Tierra K. Buck, Haoyang Li, Ruben Diaz Avalos, Sharon L. Schendel, Kathryn M. Hastie, Shane Crotty, Erica Ollmann Saphire

Science Advances

Structure of the rabies virus glycoprotein trimer bound to a prefusion-specific neutralizing antibody

Heather M. Callaway, Dawid Zyla, Florence Larrous, Guilherme Dias de Melo, Kathryn M. Hastie, Ruben Diaz Avalos, Alyssa Agarwal, Davide Corti, Hervé Bourhy, Erica Ollmann Saphire

bioRxiv

Structure of the rabies virus glycoprotein trimer bound to a pre-fusion specific neutralizing antibody

Heather M. Callaway, Dawid Zyla, Kathryn M. Hastie, Ruben Diaz Avalos, Alyssa Agarwal, Hervé Bourhy, Davide Corti, Erica Ollmann Saphire

Science

Defining variant-resistant epitopes targeted by SARS-CoV-2 antibodies: A global consortium study

Kathryn M. Hastie, Haoyang Li, Daniel Bedinger, Sharon L. Schendel, S. Moses Dennison, Kan Li, Vamseedhar Rayaprolu, Xiaoying Yu, Colin Mann, Michelle Zandonatti, Ruben Diaz Avalos, Dawid Zyla, Tierra Buck, Sean Hui, Kelly Shaffer, Chitra Hariharan, Jieyun Yin, Eduardo Olmedillas, Adrian Enriquez, Diptiben Parekh, Milite Abraha, Elizabeth Feeney, Gillian Q. Horn, Yoann Aldon, Hanif Ali, Sanja Aracic, Ronald R. Cobb, Ross S. Federman, Joseph M. Fernandez, Jacob Glanville, Robin Green, Gevorg Grigoryan, Ana G. Lujan Hernandez, David D. Ho, Kuan-Ying A. Huang, John Ingraham, Weidong Jiang, Paul Kellam, Cheolmin Kim, Minsoo Kim, Hyeong Mi Kim, Chao Kong, Shelly J. Krebs, Fei Lan, Guojun Lang, Sooyoung Lee, Cheuk Lun Leung, Junli Liu, Yanan Lu, Anna MacCamy, Andrew T. McGuire, Anne L. Palser, Terence H. Rabbitts, Zahra Rikhtegaran Tehrani, Mohammad M. Sajadi, Rogier W. Sanders, Aaron K. Sato, Liang Schweizer, Jimin Seo, Bingqing Shen, Jonne L. Snitselaar, Leonidas Stamatatos, Yongcong Tan, Milan T. Tomic, Marit J. van Gils, Sawsan Youssef, Jian Yu, Tom Z. Yuan, Qian Zhang, Bjoern Peters, Georgia D. Tomaras, Timothy Germann, Erica Ollmann Saphire

Journal of Biomolecular NMR

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution

Morgane Callon, Alexander A. Malär, Sara Pfister, Václav Římal, Marco E. Weber, Thomas Wiegand, Johannes Zehnder, Matías Chávez, Riccardo Cadalbert, Rajdeep Deb, Alexander Däpp, Marie-Laure Fogeron, Andreas Hunkeler, Lauriane Lecoq, Anahit Torosyan, Dawid Zyla, Rudolf Glockshuber, Stefanie Jonas, Michael Nassal, Matthias Ernst, Anja Böckmann, Beat H. Meier

Science Advances

A stapled peptide mimetic of the CtIP tetramerization motif interferes with double-strand break repair and replication fork protection

Anika Kuster, Nour L. Mozaffari, Oliver J. Wilkinson, Jessica L. Wojtaszek, Christina Zurfluh, Sara Przetocka, Dawid Zyla, Christine von Aesch, Mark S. Dillingham, R. Scott Williams, Alessandro A. Sartori

bioRxiv

Biomolecular solid-state NMR spectroscopy at highest field: The gain in resolution at 1200 MHz

Callon, M., Malär, A.A., Pfister, S., Rímal, V., Weber, M.E., Wiegand, T., Zehnder, J., Chávez, M., Deb, R., Cadalbert, R., Däpp, A., Fogeron, M.-L., Hunkeler, A., Lecoq, L., Torosyan, A., Zyla, D., Glockshuber, R., Jonas, S., Nassal, M., Ernst, M., Böckmann, A., Meier, B.H.

The EMBO Journal

A metabolite binding protein moonlights as a bile‐responsive chaperone

Changhan Lee, Patrick Betschinger, Kevin Wu, Dawid S Żyła, Rudi Glockshuber, James CA Bardwell

eLife

The cryo-EM structure of the human uromodulin filament core reveals a unique assembly mechanism

Jessica J Stanisich, Dawid S Zyla, Pavel Afanasyev, Jingwei Xu, Anne Kipp, Eric Olinger, Olivier Devuyst, Martin Pilhofer, Daniel Boehringer, Rudi Glockshuber

Journal of Biological Chemistry

Donor strand sequence, rather than donor strand orientation, determines the stability and non-equilibrium folding of the type 1 pilus subunit FimA

Dawid Zyla, Blanca Echeverria, Rudi Glockshuber

Science

Architecture and function of human uromodulin filaments in urinary tract infections

Gregor L. Weiss, Jessica J. Stanisich, Maximilian M. Sauer, Chia-Wei Lin, Jonathan Eras, Dawid S. Zyla, Johannes Trück, Olivier Devuyst, Markus Aebi, Martin Pilhofer, Rudi Glockshuber

Journal of Biological Chemistry

Alternative folding to a monomer or homopolymer is a common feature of the type 1 pilus subunit FimA from enteroinvasive bacteria

Dawid S. Żyła, Andrea E. Prota, Guido Capitani, Rudi Glockshuber

Structure (London, England : 1993)

The Cryoelectron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod.

Hospenthal MK, Zyla D, Costa TRD, Redzej A, Giese C, Lillington J, Glockshuber R, Waksman G

Publications

2025

Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo

Structural and Mechanistic Basis for Antibody Neutralization of the Measles Fusion Protein

Uncovering the features of Measles-targeting human antibodies elicited by the MMR vaccine

Human endogenous retrovirus K (HERV-K) envelope structures in pre- and post-fusion by cryo-EM

Structure of a SARS-CoV-2 spike S2 subunit in a pre-fusion, open conformation

Human endogenous retrovirus K (HERV-K) envelope structures in pre- and post-fusion by Cryo-EM

How herpes is assembled

2024

Intracellular Ebola virus nucleocapsid assembly revealed by in situ cryo-electron tomography

A neutralizing antibody prevents postfusion transition of measles virus fusion protein

The assembly platform FimD is required to obtain the most stable quaternary structure of type 1 pili

2023

Intracellular Ebola Virus nucleocapsid assembly revealed by in situ cryo-electron tomography.

Potent Omicron-neutralizing antibodies isolated from a patient vaccinated 6 months before Omicron emergence

Potent, Omicron-Neutralizing Antibodies Isolated From a Patient Vaccinated 6 Months Before Omicron Emergence

Structural basis for antibody-mediated neutralization of lymphocytic choriomeningitis virus

2022

A cocktail of protective antibodies subverts the dense glycan shield of Lassa virus

Structural basis for antibody-mediated neutralization of Lymphocytic choriomeningitis virus

Structure of the rabies virus glycoprotein trimer bound to a prefusion-specific neutralizing antibody

2021

Structure of the rabies virus glycoprotein trimer bound to a pre-fusion specific neutralizing antibody

Defining variant-resistant epitopes targeted by SARS-CoV-2 antibodies: A global consortium study

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution

A stapled peptide mimetic of the CtIP tetramerization motif interferes with double-strand break repair and replication fork protection

Biomolecular solid-state NMR spectroscopy at highest field: The gain in resolution at 1200 MHz

2020

A metabolite binding protein moonlights as a bile‐responsive chaperone

The cryo-EM structure of the human uromodulin filament core reveals a unique assembly mechanism

Donor strand sequence, rather than donor strand orientation, determines the stability and non-equilibrium folding of the type 1 pilus subunit FimA

Architecture and function of human uromodulin filaments in urinary tract infections

2019

Alternative folding to a monomer or homopolymer is a common feature of the type 1 pilus subunit FimA from enteroinvasive bacteria

2017

The Cryoelectron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod.